OPTIMIZATION OF Bacillus cereus GROWTH IN MEDIA WITH DIFFERENT CARBON SOURCES
Abstract
Bacillus cereus is a probiotic bacteria that requires media as a source of nutrients for its growth. This study aims to the media used were tofu liquid waste, sago liquid waste, and molasses. The method used is an experimental method, namely testing two groups of bacterial isolates; B. cereus SN7 and the consortium (combination of five isolates from B. cereus with different strains). Each group of isolates was grown with three replications on each waste medium. bacterial growth every six hours for twenty four hours using two methods; Total Plate Count (TPC) and bacterial cell biomass. There was no significant difference in growth between the two groups of bacterial isolation. The optimal growth of each isolate was found in molasses waste. The growth between two bacterial isolates in the liquid waste media was B. cereus SN7 bacterial isolate.
Keyword: Bacillus cereus, carbon, molasses, optimal growth, incubation time.
Downloads
References
Holderman, M.V., E. de Queljoe, dan S.B. Rondonuwu. (2017). Identifikasi bakteri pada pegangan eskalator di salah satu pusat perbelanjaan di kota Manado. Jurnal Ilmiah Sains, 17(1) : 13-18
Wardika, A.S., Suminto, dan A. Sudaryono. (2014). Pengaruh Bakteri Probiotik pada Pakan dengan Dosis Berbeda terhadap Efisiensi Pemanfaatan Pakan, Pertumbuhan dan Kelulushidupan Lele Dumbo (Clarias gariepinus). Journal of Aquaculture Management and Technology, 3(4): 9-17
Feliatra, F., N. Syirwani, A. Tanjung, D.S. Adithiya, M. Susanna, dan I. Lukistyowati. (2018). The Effectiveness of Heterotrophic Bacteria Isolated from Dumai Marine Waters of Riau, Used as Antibacterial against Pathogens in Fish Culture. IOP Conference Series: Earth and Environmental Science, 116 (1) : 012034.
Nainggolan, A.H. (2019). Uji Metabolit Bakteri Heterotrofik dari Muara Sungai Siak terhadap Bakteri Patogen. Skripsi. Pekanbaru. Fakultas Perikanan dan Kelatuan Universitas Riau. 61 hlm
Siregar, I.A. (2019). Efektifitas Penggunaan Bakteri Heterotrofik terhadap Pertumbuhan Ikan Nila Merah (Oreochromis niloticus). Skripsi. Pekanbaru. Fakultas Perikanan dan Kelautan Universitas Riau. 80 hlm
Nursyirwani, N., F. Feliatra, D. Yoswaty, dan R.L. Dinata. (2018). Activity of heterotrophic bacteria from marine area of Siak District against pathogenic bacteria. In IOP Conference Series: Earth and Environmental Science, 216(1) : 012047.
Feliatra, F., I. Effendi, dan S.L. Yanti. (2018). Characteristic genetics of heterotrophic bacteria in Siak River Estuary, Riau Province, Indonesia as Prospective Anti-pathogenic Bacteria to Fish and Shrimps. J Pure Appl Microbiol., 12(4) : 1801-1808
El-Sharouny, E.E., N.M.K. El-Toukhyb, N.A. El-Sersyc dan A.A. El-Aal ElGayara. (2015). Optimization and purification of mannanase produced by an alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake in Wadi El-Natron. Biotechnol. Equip., 29 (3) : 15-323
Anisah dan R. Triastuti. (2015). Media Alternatif untuk Pertumbuhan Bakteri Menggunakan Sumber Karbohidrat yang Berbeda. Seminar Nasional XII Pendidikan Biologi FKIP UNS. 855 – 860.
Wulandari, Z., E. Taufik, M. Syarif. (2017). Kajian Kualitas Produk Susu Pasteurisasi Hasil Penerapan Rantai Pendingin. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 5(3): 94- 100.
Effendi, I. (2020). Metode Identifikasi dan Klasifikasi Bakteri. Pekanbaru: Oceanum Press. 142 hlm
Nengsih, I. A., F. Feliatra, dan I. Effendi. (2020). Growth of Bacteria Bacillus cereus in Liquid Mediums with Different Carbohydrate Sources. Journal of Coastal and Ocean Sciences, 1(1) : 68-73
Endah, W., I. Tami, dan S. Ernawati. (2012). Limbah Molase: Pemanfaatan sebagai Sumber Karbohidrat untuk Perkembangbiakan Mikroorganisme. Valensi 2(5): 565-572
Septiani, W.D., A. Slamet, dan J. Hermana. (2014). Pengaruh Konsentrasi Substrat terhadap Laju Pertumbuhan Alga dan Bakteri Heterotropik. Jurnal Teknik POMITS, 3 (2) : D98 – D103.
Suastuti M. (1998). Pemanfaatan Hasil Samping Industri Pertanian Molase dan Limbah Cair Tahu sebagai Sumber Karbon dan Nitrogen untuk Produki Biosurfaktan oleh Bacillus sp Galur Komersil dan Lokal. Tesis. Program Pascasarjana Institut Pertanian Bogor. Bogor
Alviolita, C. (2017). Pengaruh Pemberian Tetes Gula (Molase) Sebagai Pupuk Alami Terhadap Kelimpahan Nannochloropsis Sp. Skripsi, Universitas Brawijaya.
Copyright (c) 2021 Asian Journal of Aquatic Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors submitting manuscripts should understand and agree that the copyright of manuscripts of the article shall be assigned/transferred to the "Asian Journal of Aquatic Sciences". This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International License, where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform or build upon the material, you must distribute your contributions under the same license as the original.
