TOXICITY OF SEA GRASS EXTRACT (Eucheuma cottonii AND Gracillaria sp) TO LARVA Artemia salina
Abstract
Seaweed is a source of foreign exchange, namely as a main export product and a source of income for coastal communities. Previous research on species Eucheuma cottonii and Gracillaria sp contain bioactive compounds that can be used in medicine, for example as anti-cancer. The purpose of this study was to determine the LC50 value and safe concentration of Artemia salina larvae. The research was conducted at the Chemical Oceanography Laboratory of the Department of Marine Sciences, Faculty of Fisheries and Marine, Riau University in July 2020. The method used in this research is the experimental method. The results of the research on the toxicity of the n-hexane extract of seaweed E. cottonii and Gracillaria sp had bioactivity compounds against A. salina shrimp larvae, indicated by a small LC50 value (<1000 ppm), namely 62.62 ppm for E. cottonii and 83.55. ppm for Gracillaria sp, so it is included in the toxic category. According to research by experts, if the extract or compound tested is less than 1000 ppm, it is considered that there is biological activity. For a safe concentration for the survival of A. salina, 6.262 ppm for E. cottonii and 8.355 ppm for Gracillaria sp.
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References
Khurniasari, D.W. (2004). Potensi Antikanker Senyawa Bioaktif Ekstrak Kloroform dan Metanol Makroalgae Sargassum duplicatum J. Agardh. Fakultas Biologi Universitas Gadjah Mada Jogjakarta. Jogjakarta
Krishnaraju, A. V., T. V Rao., D. Sundararaju., M. Vanisree., H-S. Tsay., and G. V. Subbaraju. (2006). Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering, 3: 125 – 135.
Colegate, S.M. and R.J. Molyneux. (2007). Bioactive Natural Products: Determination, Isolation and Structural Determination Second Edition. Prancis: CRC Press.
Hiola, R., R. Tuiyo dan Syamsuddin. (2014). Pengaruh Salinitas yang Berbeda terhadap Penetasan Kista Artemia sp. di Balai Benih Ikan Kota Gorontalo Provinsi Gorontalo. Jurnal Ilmiah Perikanan dan Kelautan, 2 : 52 – 55.
Harmita dan M. Radji. (2008). Analisis Hayati. Penerbit Buku Kedokteran. Jakarta.
Tyas. (2016). Uji Toksisitas Letal Cr6+ terhadap Ikan Nila (Oreochromis niloticus). Fakultas Perikanan dan Ilmu Kelautan. Jurnal Ilmu Pertanian Indonesia, 2: 128 - 132.
Sangi, M., L. Momuat, dan M. Kumaunang. (2012). Uji Toksisitas dan Skrining Fitokimia Tepung Gabah Pelepah Aren (arenga pinnata). Jurnal Ilmiah Sains, 12: 128-134.
Nedi, S., Thamrin., dan H. Marnis. (2006). Toksisitas Deterjen terhadap Benih Ikan Kakap Putih (Lates calcarifer, Bloch). Berkala Perikanan Terubuk, 33: 75 – 81.
Meyer, B.N., N.R. Ferrigni, J.E. Putnan, L.B. Jacobsen, D.E. Nicholas, and J.L. Mclaughlin. (1982). Brine Shrimp: A Conveinient General Bioassay For Active Plant Constituents, Planta Medica. 4 : 31 - 34.
Nur, A. dan Y. Zuhud. (2011). Kanker Lenyap Berkat Sirsak. Jakarta: PT. Agro Media Pustaka.
Sharo. M. N., Ningsih, Rachmawati., A. Nasichuddin, Ahmad, dan Hanapi. (2013). Uji Toksisitas dan Identifikasi Senyawa Ekstrak Alga Merah (Eucheuma cottonii) Terhadap Larva Udang Artemia salina Leach. Jurnal Alchemy, 3: 170 - 177.
Sukardiman., R. Abdul dan P. N. Fatma. (2004). Uji Praskrining Aktivitas Antikanker Ekstrak Eter dan Ekstrak Metanol Marchantia planiloba Steph. dengan Metode Uji Kematian Larva Udang dan Profil Densitometri Ekstrak Aktif. Majalah Farmasi Airlangga, 4: 97 - 100.
Astuti, P., S. Utami, T. Pratiwi, T. Hertiani, G. Alam, A. Tahir and S. Wahyono. (2005). Antimicrobial Activity Screening of Marine Sponges Extracts Colected from Barang Lomposea. Journal of Traditional Medicine,10:32.
Hardiko, F. (2018). Toksisitas Ekstrak Rumput Laut (Eucheuma cottonii) dengan Pelarut n-Heksana dan Etil Asetat terhadap Artemia salina. Fakultas Perikanan dan Kelautan Universitas Riau.
Suryati, E. (2015). Uji Ekstrak Ramuan “Kandungan Subur” (Kunyit (Curcuma domestica Val.), Kencur (Kaempferia galanga L.), Adas (Foeniculum vulgare Mill.) dan Pegagan (Centella asiatica)) pada Berbagai Pelarut terhadap Toksisitas Larva A. salina. Fakultas Sains dan Teknologi Universitas Islam Negri Maulana Malik Ibrahim, Malang.
Kunsah, B. dan R. Widyastuti. (2018). Uji Toksisitas Akut Kulit Kentang (Solonum tuberosum L.) terhadap Larva A. salina Leach dengan Metode BSLT (Brine Shrimp Lethality Test). Prodi D3 Analisis Kesehatan Universitas Muhammadiyah, Surabaya.
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