EFFECT OF rGH HORMONE ADMINISTRATION ON PROTEASE ENZYME ACTIVITY AND RNA/DNA RATIO OF STRIPED CATFISH (Pangasianodon hypophthalmus) MAINTAINED IN SALINE MEDIA
Abstract
Striped catfish is a prevalent fish and is in great demand. Striped catfish cultivation generally uses fresh water as a living medium, but several freshwater fish commodities are starting to be developed to adapt to saline media. Harvesting striped catfish usually takes approximately 3 - 8 months. To overcome this, one way is to use growth hormone. The growth hormone currently used is Recombinant Growth Hormone (rGH). GH administration has been reported to have various effects, particularly on protein metabolism. In fish digestion, protein from feed cannot be directly absorbed but is first broken down by protease enzymes into amino acids. The physical appearance, which is the phenotype of an organism, is the result of metabolic processes in each cell that makes up the organism. This research aimed to analyze the effect of administering the rGH hormone on protease enzyme activity, RNA/DNA ratio, and growth of striped catfish maintained in saline media. The method used was experimental in the form of a Completely Randomized Design (CRD) with four treatments and three replications. Based on the results obtained during the research, it shows that Protease Enzyme Activity (U/minute) and the best RNA/DNA Ratio use a dose of 3 mg/kg with respective values of 0.75±0.24 U/minute and3.30±0.49.
Downloads
References
Tahe, S., Nawang, A., Suwoyo, H.S. Pemasyarakatan Teknologi Budidaya Udang Vaname (Litopenaeus vanname) Sistem Polikultur dengan Ikan Bandeng (Chanos chanos) di Tambak Salnitas Rendah. Prosiding Forum Inovasi Teknologi Akuakultur, 2011; p10.
Fitrani, M. Rekayasa Lingkungan Budidaya untuk Meningkatkan Kualitas Ikan Patin Siam (Pangasius hypopthalmus): Peran Salinitas. Sekolah Pascasarjana Institut Pertanian Bogor, 2009; p 83.
Fissabela, F.A., Suminto, S., Ristiawan, A.N. Pengaruh Pemberian Recombinant Growth Hormone (rGH) dengan Dosis Berbeda pada Pakan Komersial terhadap Efisiensi Pemanfaatan Pakan, Pertumbuhan dan Kelulushidupan Benih Ikan Patin (P. pangasius). Sains Akuakultur Tropis, 2017; 1(1): 1-9.
Alimuddin, A., Handoyo, B., Utomo, N.B.P. Efektivitas Pemberian Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang (Epinephelus lanceolatus, Bloch 1790) melalui Perendaman dan Oral terhadap Pertumbuhan Elver Ikan Sidat (Anguilla bicolor bicolor). Iktiologi Indonesia, 2014; 14 (3): 179-189.
Pamungkas, W. Aktivitas Osmoregulasi, Respons Pertumbuhan dan Energetic Cost pada Ikan yang Dipelihara dalam Lingkungan Bersalinitas. Media Akuakultur, 2012; 7 (1): 44-51
Taufik, I., Kusrini, E. Peran Hormon dan Syaraf pada Osmoregulasi Hewan Air. Media Akuakultur, 2006; 1(2) : 81-85
Masuda, Y., Oku, H., Okumura, T., Kurokawa, T. Feeding Restriction Alters Expression of some ATP Related Genes More Sensitively than the RNA/DNA Ratio in Zebrafish (Danio rerio). Comp Biochem Physiol B Biochem Mol Biol., 2009; 152: 287-291
Nirmala, K., Lesmono, D.P., Djokosetiyanto, D. Pengaruh Teknik Adaptasi Salinitas terhadap Kelangsungan Hidup dan Pertumbuhan Benih Ikan Patin, Pangasius sp. Akuakultur Indonesia, 2005; 4(1) : 25-30
Riswan, M., Henni, S., Usman, M.T. Respon Fisiologis dan Histopatologis Ikan Patin Siam (Pangasianadon hypopthalmus) yang Dipelihara pada Salinitas Berbeda. Asian Journal of Aquatic Sciences, 2022; 5(1) : 10-19
Kurniawan, A., Basuki, F., Nugroho, R.A. Pengaruh Pemberian Rekombinan Hormon Pertumbuhan (rGH) melalui Metode Oral dengan Interval Waktu yang Berbeda terhadap Pertumbuhan dan Kelulushidupan Benih Ikan Bawal Air Tawar (Colossoma macropomum). Journal of Aquaculture Management and Technology, 2018; 6(3): 20-29.
Ratnawati, P., Arfah, H., Sudrajat, A.O. Growth and Survival of giant Gourami Juvenile Immersed in Freshwater Containing Growth Hormone. Jurnal Akuakultur Indonesia, 2012; 11(2): 162-167.
Bergmeyer H.V., Grassl, M.G. Determination with Glucose Oxidize and Peroxidase: Methods of Enzymatic Analysis. 2nd edition. Weinheim, 1983.
Pamungkas, W., Nurlaela, I., Darmawan, J. Analisis Rasio RNA/DNA Ikan Patin Siam (Pangasianodon hypophthalmus) F-2 Tumbuh Cepat Hasil Seleksi. Prosiding Forum Inovasi Teknologi Akuakultur, 2015; 251-258.
Manik, R.R.D.S, Jogi, J. Nutrisi dan Pakan Ikan. Widina Bhakti Persada Bandung, 2021, p 99.
Fauziah, S., Algadri, A,, Untung, S., Slamet, P. Aktivitas Protease dan Amilase Pada Hepatopankreas dan Intestine Ikan Nilem (Osteochilus hasselti) C.V. Scripta Biologica, 2014; 1(1): 43-48
Devlin, R.H., Biagi, C.A., Yesaki, T.Y. Growth, Viability and Genetic Characteristics of GH Transgenic Coho Salmon Strains. Aquaculture, 2004; 236 (1): 607-632.
Syahrir, M., Kantun, W., Cahyono, I. Kinerja Enzim Pencernaan Ikan Nila Salin (Oreochromis niloticus) Berdasarkan Lingkungan Budidaya. Fisheries Journal, 2020; 3(1): 42-55.
Manurung, R.E.C. Pengaruh Pemberian Dosis Hormon rGH pada Kadar Protein Pakan Berbeda terhadap Pertumbuhan dan Kelangsungan Hidup Ikan Koi (Cyprinus carpio). Fakultas Pertanian. Universitas Sumatera Utara, 2021; p 116.
Silalahi, E.M., Tang, U.M., Mulyadi, M. The Effect of Different Doses of rElGH (Rekombinat Ephinephelus lanceolatus Growth Hormone) on Growth and Survival of Pomfret Fish in Recirculation Systems. Jurnal Online Mahasiswa Fakultas Perikanan dan Ilmu Kelautan Universitas Riau, 2017; 4 (2): 1-9
Chícharo, M.A., Chícharo, L. RNA: DNA ratio and other nucleic acid derived indices in marine ecology. International Journal of Molecular Sciences, 2008; 9(8): 1453-1471.
Suhermiati, I. Analisis Miskonsepsi Siswa pada Materi Pokok Sintesis Protein ditinjau dari Hasil Belajar Biologi Siswa. Berkala Ilmiah Pendidikan Biologi (BioEdu), 2015; 4(3).
Gusrina, G. Genetika dan Reproduksi Hewan. Deep Publish, 2018; p597.
Jamal, K. Pengaruh Pengkayaan Rotifer dan Artemia dengan Beta Karoten pada Pemeliharaan Larva Rajungan (Portunus pelagicus). Program Studi Magister Ilmu Perikanan. Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin, 2019; p 54.
Hasbullah, D., Karim, M.Y., Zainuddin, Z., Trijuno, D.D. The Effect of Salinity on the Body Chemical Composition and RNA/DNA Ratio of the Hybrid Brackishwater Nile tilapia (Oreochromis niloticus). AACL Bioflux, 2018; 11(3): 943-947
Faidar, S., Budi, B., Indrawati, E. Analisis Pemberian Vitamin C pada Rotifer dan Artemia terhadap Sintasan, Rasio RNA/DNA, Kecepatan Metamorfosis dan Ketahanan Stres Larva Rajungan (Portunus pelagicus) Stadia Zoea. Journal of Aquaculture and Environment, 2020; 2(2): 30-34
Sari, E.P., Mokoginta, I., Jusadi, D. Pengaruh Pemberian Kromium Ragi dalam Pakan terhadap Kinerja Pertumbuhan Ikan Baung (Hemibagrus nemurus). Ilmu-ilmu Perairan dan Perikanan Indonesia, 2008; 16(1): 17-23
Dewi, R.R.S.P.S., Tahapari, E. Performa Ikan Lele Afrika (Clarias gariepinus) Hasil Seleksi terhadap Pertumbuhan, Sintasan, Konversi Pakan, Rasio RNA/DNA dan Nilai Bioekonomi. Media Akuakultur, 2017; 12(1): 11-17
Copyright (c) 2024 Mohamad Ardani Syahputra, Usman M Tang, Henni Syawal
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors submitting manuscripts should understand and agree that the copyright of manuscripts of the article shall be assigned/transferred to the "Asian Journal of Aquatic Sciences". This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International License, where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform or build upon the material, you must distribute your contributions under the same license as the original.
