ISOLATION OF Escherichia coli FROM VANAME SHRIMP (Litopenaeus vannamei) POND AND THE SENSITIVITY TOWARD ANTIBIOTICS
Abstract
Vannamei shrimp (Litopenaeus vannamei) is a fishery commodity widely cultivated in various parts of Indonesia. The main problem often found in vannamei shrimp production is poor water quality during the rearing period. This study aims to isolate and identify E. coli contained in water, sediment, and meat of vannamei shrimp cultivated in shrimp ponds and examine its sensitivity to antibiotics. The research was conducted from March to May 2023 at the Technical Implementation Unit (UPT) Vannamei Shrimp Ponds of the Maritime Affairs and Fisheries Service, Tanjung Punak Village, Rupat Utara District, Bengkalis Regency, Riau. The survey method was used in this study. The Most Probable Number (MPN) method was employed for E. coli isolation to calculate bacterial density, and the Kirby-Bauer method was used to test bacterial sensitivity to antibiotics. Based on the identification of the six isolates carried out, it was found that only two isolates were positively identified as E. coli bacteria, namely SD C 3.1 and IP B 1.1, which were isolated from sediment and the Wastewater Treatment Plant (WWTP) pond. The diameter of the inhibition zone for sediment isolate RF01 against the antibiotic chloramphenicol was 18.75 mm, indicating intermediate sensitivity. On the other hand, WWTP isolates RF02 exhibited an inhibition zone of 17 mm for chloramphenicol, indicating medium sensitivity as well. However, it showed resistance to penicillin with a diameter of 6.5 mm and resistance to ampicillin with a diameter of 2 mm
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References
Arsad, S., Afandy, A., Purwadhi, A.P., Maya V, B., Saputra, D.K., Buwono, N.R. Studi Kegiatan Budidaya Pembesaran Udang Vaname (Litopenaeus vannamei) dengan Penerapan Sistem Pemeliharaan Berbeda. Jurnal Ilmiah Perikanan dan Kelautan, 2017; 9: 1–5.
Sa’adah, W., Milah, K. Permintaan Udang Vannamei (Litopenaeus vannamei) di Kelompok Pembudidaya Udang At-Taqwa Paciran Lamongan. Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis, 2019; 5(2): 243–251.
Khairunnida, G.R., Rusmini, H., Maharyun, E. Isolasi dan Identifikasi Bakteri Escherichia coli Penyebab Waterborne Disease pada Air Minum Kemasan dan Isi Ulang. Jurnal Ilmiah Kesehatan Sandi Husada, 2020; 9: 634–639.
Wahyuni, Y., Jamilah, I., Suryanto, D. Isolasi Bakteri Patogen Oportunistik dari Tambak Udang Sumatera Utara. Jurnal Agrohita, 2017; 1: 71–75.
Alghifari, D., Kuswandi, B., Pratoko, D.K. Pengembangan Sensor Kloramfenikol Berbasis Imobilisasi Bovine Serum Albumin (BSA) pada Selulosa Asetat dengan Metode Spektroflorometri. Jurnal Pustaka Kesehatan, 2017; 5(1): 40–45.
Rahmaniar, R.P., Widhowati, D., Hidayah, N. Sensitivitas Antimikroba terhadap Bakteri Escherichia coli yang Diisolasi dari Udang di Pasar Keputran Surabaya. Jurnal Kajian Veteriner, 2019; 7(2): 93–100.
Intan, K., Diani, A., Suci, A., Nurul, R., Barat, J. Aktivitas Antibakteri Kayu Manis (Cinnamomum burmanii) terhadap Pertumbuhan Staphylococcus aureus Karlina. Jurnal Kesehatan Perintis Journal, 2021; 8(2), 121–127.
Sapitri, A., Afrinasari, I. Identifikasi Escherichia coli pada Cincau yang Dijual di Pasar Baru Stabat. Journal of Pharmaceutical and Sciences, 2019; 2(2): 18–23.
[CLSI] Clinical and Laboratory Standard Institute. Performance Standards for Antimicrobial Susceptibility Testing. Ed. CLSI supplement M100. Wayne, PA, 2020.
Sahrijanna, A., Septiningsih, E. Variasi Waktu Kualitas Air pada Tambak Budidaya Udang dengan Teknologi Integrated Multitrophic Aquaculture (IMTA) di Mamuju Sulawesi Barat. Jurnal Ilmu Alam dan Lingkungan, 2017; 8(16): 52–57.
Nurhasanah., Junaidi, M., Azhar, F. Tingkat Kelangsungan Hidup dan Pertumbuhan Udang Vaname (Litopanaeus vannamei) pada Salinitas 0 ppt dengan Metode Aklimatisasi Bertingkat Menggunakan Kalsium CaCO3. Jurnal Perikanan, 2021; 11(2): 166–177.
Suhendar, D.T., Zaidy, A.B., Sachoemar, S.I. Profil Oksigen Terlarut, Total Padatan Tersuspensi, Amonia, Nitrat, Fosfat dan Suhu pada Tambak Intensif Udang Vanamei. Jurnal Akuatek, 2020; 1(1): 1–11.
Jamilatun, M., Aminah, A. Isolasi dan Identifikasi Escherichia coli pada Air Wudhu di Masjid yang Berada di Kota Tangerang. Jurnal Medikes, 2016; 3(1): 81–90.
Susilo., Setyaningsih, M., Mulyawati, D. Strain Escherichia coli dari Usus Ayam: Karakterisasi Profil Resistensi Antibiotika Ciprofloxacin dan Erythromycin. Jurnal Pembelajaran dan Biologi Nukleus, 2022; 8(1): 103–113.
Trisno, K., Suarjana, I.G.K. Isolasi dan Indentifikasi Bakteri Escherichia coli dari Udara pada Rumah Potong Unggas Swasta di Kota Denpasar. Indonesia Medicus Veterinus, 2019; 8(5): 685–694.
Rahayu, W.P., Nurjanah, N., Komalasari, E. Escherichia coli: Patogenitas, Analisis dan Kajian Resiko. IPB Press. Kota Bogor, 2018.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, W., Stanley, T. Bergey's Manual of Determinative Bacteriology. 9th edition. Baltimore: Williams and Wilkins, 1994.
Dian, R., Fatimawali, F., Budiarso, F. Uji Resistensi Bakteri Escherichia coli yang Diisolasi dari Plak Gigi terhadap Merkuri dan Antibiotik Kloramfenikol. Jurnal E-Biomedik (EBM), 2015; 3(1): 59–63.
Virgianti, S.E., Moelyaningrum, A.D., Ningrum, P.T. Kandungan Residu Kloramfenikol pada Udang Putih (Litopenaeus vannamei). Buletin Kesehatan Lingkungan Masyarakat, 2022; 41(4), 149–155.
Yusriyani, Y., Asfi, D., Yusliati, R.K. Uji Daya Hambat Ekstrak Etanol Daun Miana Merah (Coleus benth) terhadap Staphylococcus Aureus. Jurnal Kesehatan Yamasi Makassar, 2023; 7(1): 10–16.
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