TEST THE ABILITY OF SEDIMENT BACTERIA ISOLATES IN DEGRADATING PHENOL

  • Siska Monika University of Riau
  • Dessy Yoswaty Universitas Riau
  • Nursyirwani Nursyirwani Universitas Riau
Keywords: Sediment Bacteria, Degradation, Phenol

Abstract

Phenol degrading bacteria can be found in various habitats in marine environments. This study aims to obtain bacteria from sediments that are able to degrade phenol. The process of bacterial purification and degradation was carried out from August to September 2018 at the Marine Microbiology Laboratory, Department of Marine Sciences, Faculty of Fisheries and Marine, University of Riau. Analysis of the reduction in phenol concentration was carried out using the APHA 5530 method using UV-VIS spectrophotometry conducted at the Health and Environment Laboratory. The bacterial isolates used as test bacteria were isolates BF1A, BF4B and BF9C. Bacterial and biochemical tests were carried out for all bacterial isolates. Two isolated showed mehyl red negative, all isolates were motile. Three isolates were positive catalase, able to ferment glucose and sucrose fermented citrate and two isolat were Gram negative bacterial. The three bacterial isolates were able to degrade phenol with the highest degradation for 1ppm shown in isolates BF1A, the highest degradation of concentrations of 2 ppm and 3 ppm was shown in isolates BF9C. Thus, the isolat BF9C was able to degrade the highest phenol.

Downloads

Download data is not yet available.

References

Dewilda, Yommi. Reri Afrianita and Fano Fadhilah I. 2012. Degradasi senyawa fenol oleh mikroorganisme laut.Jurnal Teknik Lingkungan UNAND 9(1):59-73.

Dwipayana dan Ariesyady. 2005.Identification Of Bacterial Diversity In Waste Recycling Paint Sludge By Conventional Microbiological Technique. Environmental Engineering Study Program.Bandung:ANDI OFSEET.Ali. A. 2005. Mikrobiologi Dasar. Makasar. Universitas Negeri Makasar Press.

Hank, D. N. Saidani, A. Namane and A. Hella. 2010. Batch Phenol Biodegradation Study and Application of Factorial Experimental Design. Journal of Engineering Science and Technology Review 3(1), 123-127.

Haryani Y, Chainullfifah and Rustiana. 2012. Fermentasi Karbohidrat oleh Isolat Salmonella spp. J. ind .Che. Acta. 3(1):23-26.

Khusnuryani, A.2015. Karakterisasi Bakteri Pendegradasi Fenol Dan Pembentuk Biofilm Dari Sumber Alami Dan Artifisial. Kaunia 9(1): 40-50.

Mohite, B.V., R.E, Jalgaonwala, S, Pawar, and A.,Morankar. 2010. Isolation and Characterization of Phenol Degrading Bacteria From Oil Contaminated Soil. Research Article: Innovative Romanian Food Biotechnology,7: 61-65

Murray. 2005. Buku Ajar Mikrobiologi. Penerbit Buku Kedokteran EGC: Jakarta.

Nursaadah, Santosa D. A, Suhartono T. M. (2000). Karakterisasi Bakteri Pendegradasi Fenol Asal Danau Buntal Kalimantan Tengah. Jurnal ilmutanah dan lingkungan. 3(2):24-31.

Piakong, M.T. 2006. The Performance of Phenol Biodegradation by Candida tropicalis RETL-Cr1 Using Batch and Fed-batch Fermentation Techniques. Ph.D Thesis. Universiti Teknologi Malaysia, Skudai.

Slamet. 2008. Degradasi Senyawa Fenol dengan Metode Fotokatalisis Menggunakan Reaktor Annular UV-C. [Online]. Tersedia: http://staff.ui.ac.id/ internal/132048271/publikasi /Prosiding Seminar Nasional Rekayasa Kimiadan Proses 2008 Teknik Kimia UNDIP.pdf [28 Januari 2018].

Suhaila, N. 2010. Optimization of Parameters for Phenol Degradation by Rhodococcus UKM-P in Shake Flask Culture. Proceeding of the World Congress on Engineering. (1)
Published
2020-01-10
How to Cite
Monika, S., Yoswaty, D., & Nursyirwani, N. (2020). TEST THE ABILITY OF SEDIMENT BACTERIA ISOLATES IN DEGRADATING PHENOL. Asian Journal of Aquatic Sciences, 2(1), 79-84. https://doi.org/10.31258/ajoas.2.1.79-84
Section
Articles