ANALYSIS OF Escherichia coli BACTERIA CONTENT IN FISH MEAT WITH VITEK 2 COMPACT
Abstract
Transmission of Escherichia coli in food can occur through contact with infected workers, as well as tools and materials used during food processing. One way to determine the presence of E.coli in processed fish balls is to perform a bacteriological test. The purpose of this study was to detect the presence or absence of bacteria in fish balls so that it can be seen whether these meatballs are in the hygienic category and are suitable for consumption or not. This type of research is a laboratory experiment. This research was conducted at the Maluku Provincial Health Laboratory on 26-28 January 2022. The sample used in this study was fish balls taken by purposive sampling (taken according to research needs) for laboratory testing. Based on the results above, it shows that the E.coli bacteria found in the meatball samples during the phenotyping test with Vitek 2 compact both at a dilution of 10-1 to 10-3 were not identified because they had not passed the maximum limit. Based on the results obtained, it was found that the fish balls made by service participants in Mamala country were still in accordance with the criteria of the Indonesian National Standard (SNI) No.7388:2009 regarding the maximum limit of E.coli bacterial contamination in processed meat products made by the government for the protection of consumers regarding the quality of the product in circulation and declared to meet the requirements for the maximum threshold of microbial contamination in food if the value is 3/g for the status fit for consumption. The condition of the value of the test results of the fish balls sample under study is due to the fact that food makers or processors when processing fish balls still use the correct sanitation and hygiene principles and according to procedures
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References
Zain, R., Hidanah, S., Damayanti, R., Warsito, S.H. (2021). Detection of Salmonella sp. on Bulk Meatballs and Packaged Meatballs at Sepanjang Market, Sidoarjo. Journal of Applied Veterinary Science And Technology, 2(2), 31. https://doi.org/10.20473/javest.v2.i2.2021.31-36
Patang. (2017). The Making Meatballs Based Main Milk Fish with Addition of Small Crab. The International Journal of Science & Technoledge, 5(7), 41–44.
Poluakan, O.A., Dien, H.A., Ijong, D.G. (2015). Mutu Mikrobiologis Bakso Ikan yang Direndam Asap Cair, Dikemas Vakum, Dipasteurisasi dan Disimpan pada Suhu Dingin. Media Teknologi Hasil Perikanan, 3 (2)
Yanti, N.P.Y.C., Sudarmanto, I.G., Sarihati, I.G.A.D. (2021). Gambaran Angka Lempeng Total dan Identifikasi Escherichia coli pada Bakso Ayam yang Dijual di Desa Sanur Kauh Denpasar Selatan. Mediatory, 9(2), 2338–1159.
Djodjoka, J.A., Nancy, S.H., Malonda, Maureen, I., Punuh. (2015). Identifikasi Bakteri Escherichia coli pada Jajanan Bakso Tusuk di Sekolah Dasar Kota Manado. http://fkm.unsrat.ac.id
Effendi, I., Tanjung, C.F., Elizal. (2018). Growth of Heterotrophic Bacteria in Sea Water Contaminated with Rinso Detergent. Asian Journal of Aquatic Sciences, 1(1): 40–44.
Islam, M.M., Islam, M.N., Sharifuzzaman, Fakhruzzaman, M. (2014). Isolation and identification of Escherichia coli and Salmonella from meat and liver of local and imported chicken. International Journal of Natural and Social Sciences, 1, 1–7.
Anggraini, D., Hasanah, U., Savira, M., Andrini, F., Irawan, D., Prima, R. (2018). Prevalensi dan Pola Sensitivitas Enterobacteriaceae Penghasil ESBL di RSUD Arifin Achmad Pekanbaru. Jurnal Kedokteran Brawijaya, 30(1): 47–52.
Suardana, I. Utama, I.H., Wibowo, M.H. (2014). Identifikasi Escherichia coli O157:H7 Dari Feses Ayam dan Uji Profil Hemolisisnya pada Media Agar Darah. Jurnal Kedokteran Hewan, 8: 1–8
Jamilatun, M., Aminah, A. (2016). Isolasi dan Identifikasi Escherichia coli pada Air Wudhu di Masjid yang Berada di Kota Tangerang. Jurnal Medikes (Media Informasi Kesehatan), 3(1): 81–90. https://doi.org/10.36743/medikes.v3i1.154
Liempepas, A., Lolo, W.A., Yamlean, P.V.Y. (2019). Isolasi dan Uji Antibakteri dari Isolat Bakteri yang Berasosiasi dengan Spons Callyspongia aerizusa serta Identifikasi Secara Biokimia. Pharmacon, 8(2), 380. https://doi.org/10.35799/pha.8.2019.2930
Rahayu, A.S., Gumilar, M.H. (2017). Uji Cemaran Air Minum Masyarakat Sekitar Margahayu Raya Bandung Dengan Identifikasi Bakteri Escherichia coli. Indonesian Journal of Pharmaceutical Science and Technology, 4(2), 50. https://doi.org/10.15416/ijpst.v4i2.13112
Wally, P., Abdollah, A. (2022). Pemberdayaan Perempuan Melalui Pelatihan Pembuatan Bakso Ikan Cakalang bagi Masyarakat Negeri Mamala Kabupaten Maluku Tengah. 2(April), 75–84.
Wally, P., Abdollah, A., Marwah, A.S., Sohilauw, I.S.S., Wahyudi, A. (2022). Pelatihan Pembuatan Bakso Sehat Bagi Ibu Rumah Tangga Desa Mamala Kabupaten Maluku Tengah. Program Studi Pendidikan Biologi, FKIP Universitas Muhammadiyah Maluk. 2(1), 31–42.
Mayaserli, D. P., & Anggraini, D. (2019). Identifikasi Bakteri Escherichia coli pada Jajanan Bakso Tusuk di Sekolah Dasar Kecamatan Gunung Talang. Jurnal Kesehatan Perintis (Perintis’s Health Journal), 6(1): 30–34. https://doi.org/10.33653/jkp.v6i1.220
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