EFFECTS OF BIOFLOC AND PHOTOPERIOD MANIPULATION COMBINATION ON BLOOD STRUCTURE OF Pangasianodon hypophthalmus
Abstract
Pangasianodon hypopthalmus can be reared under biofloc system and short photoperiod. To understand the effect of biofloc system and photoperiod manipulation to wards blood structure of P. hypopthalmus a study was conducted on September to December 2020. This experiment using a factorial CRD design with 2 level for B factor (biofloc and no biofloc), 3 level for P factor (Natural, 18 hours dark, 24 hours dark), 3 repetition. The fish was reared in 110L tank, 30 fishes/tank, fed with fish pellet (FF-999, Provit 781-1 and Provit 781-2). Parameters measured were the number of erythrocytes, leukocytes, the level of hematocrit and leukocrite and leukocyte types. The blood was collected using syringe wetted with EDTA 10%. The number of blood cells was calculated using haemocytometer, while the white blood cells were stained using Giemsa solution and checked under a binocular microscope. Results shown that there is no different blood structure in fish of all treatments. Total erythrocytes are 1,681,111-2,647,777 cell/mm3, total leukocytes are 55.031-55.655 cell/mm3, hematocrit 20.33-38.11% and leukocrite 20.33-38.11%. White corpuscle are basophil 00-3.89%, eosinophils 0-3.67%, lymphocytes 26.67-73.89%, trombosit 1-48.89%, monocytes 13.33-30.22% and neutrophill 0.00-9.22%. Based on data obtained, it can be concluded that the biofloc system and photoperiod manipulation combination do not negatively affects the fish hematology.
Downloads
References
Sunarma, A. (2007). Panduan singkat teknik pembenihan ikan patin Pangasius hypophthalmus. Balai Besar Perikanan Budidaya Air Tawar Sukabumi.
Suprapto, Samtafsir SL. (2013). Biofloc 165 Rahasia Sukses Teknologi Budidaya Lele. Depok: AGRO 165.
Najiah, M., Nadirah M., Marina H., Lee, S. W., Nazaha W.H. (2008). Quantitative comparisons of erythrocyte morphology in healthy freshwater fish species from Malaysia. Res. J. of Fisheries and Hydrobiology, 3(1): 32-35
Prasetio, E., Fakhrudin, M., Hasan, H. (2017). Pengaruh Serbuk Lidah Buaya (Aloe vera) Terhadap Hematologi Ikan Jelawat (Leptobarbus hoevenii) yang Diuji Tantang Bakteri Aeromonas hydrophila. Jurnal Ruaya, 5(2): 44-54.
Riantono, F., Kismiyati, L. Sulmartiwi. (2016). Perubahan Hematologi Ikan Mas Komet (Carassius auratus) Akibat Infestasi Argulus japonicus Jantan dan Argulus japonicus Betina. Journal of Aquaculture and Fish Health, 5(2): 49
Copyright (c) 2022 Yuli Anggreani Putri Br Surbakti, Windarti Windarti, Eddiwan Eddiwan
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors submitting manuscripts should understand and agree that the copyright of manuscripts of the article shall be assigned/transferred to the "Asian Journal of Aquatic Sciences". This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International License, where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform or build upon the material, you must distribute your contributions under the same license as the original.
